Development of diploid and triploid interspecific hybrids betweenLilium longiflorumandL. concolorby ovary slice culture

摘要

AbstractOvary slice culture, after cut‐style pollination, was used to develop interspecific hybrids betweenLilium longiflorumandL. concolor.Reciprocal crosses between diploid cultivars (2n = 2x = 24) were carried out. On the days 30, 35, 40 and 45th after pollination (DAP), ovaries were sliced and cultured on a modified hormone‐free Murashige‐Skoog (M–S) medium without NH4NO3, supplemented with 6 sucrose, 50 mg/1 yeast extract and 0.25 gelrite at pH 6.3. For theL. longiflorum × L. concolorcross, ovule germination was found to be best at 30 DAP. After transfer to a M–S (half‐strength) medium supplemented with 1.5 sucrose and 0.25 gelrite at pH 5.8, diploid and triploid hybrid plants were established. In contrast, ovules from theL. concolor × L. longiflorumcross did not germinate. The hybridity of the plantlets obtained was verified by karyotype and isozyme analysis. The importance of the ovary slice culture technique as a tool to develop new hybrids between incompatible lilly plants