ABO blood grouping of human bloodstains was performed by a sandwich ELISA using a species-specific monoclonal antibody to the amino-terminal cytoplasmic domain of human red cell membrane band 3. In a blind trial, all A, B and O bloodstains (a 1 cm long thread) and AB bloodstains (a 1.5 cm long thread) were accurately typed by this method. Even when bloodstains were contaminated by other body fluids (e.g., semen and saliva), only the ABO blood group epitopes on band 3 of the red cell membrane were detected. Thus, identification of human blood and ABO blood grouping of bloodstains which were contaminated by other body fluids could be simultaneously performed by this method.
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机译:使用针对人红细胞膜条带 3 氨基末端细胞质结构域的物种特异性单克隆抗体,通过夹心 ELISA 对人血迹进行 ABO 血型分析。在盲法试验中,所有 A、B 和 O 血迹(1 厘米长的线)和 AB 血迹(1.5 厘米长的线)都通过这种方法准确分型。即使血迹被其他体液(例如精液和唾液)污染,也只检测到红细胞膜第 3 条上的 ABO 血型表位。因此,该方法可以同时进行被其他体液污染的人血和ABO血型的鉴定。
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