Methods of metabolic flux analysis (MFA) provide insights into the theoretical capabilities of metabolic networks and allow probing the in vivo performance of cellular metabolism. In recent years, an increasing awareness has developed that network analysis methods within the systems biology toolbox are serving to improve our understanding and ability to manipulate metabolism. In this minireview the potential of MFA to increase the chances of success in metabolic engineering of plants is presented, recent progress related to engineering and flux analysis in central metabolism of plants is discussed, and some recent advances in flux analysis methodology are highlighted.
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