Young rolled leaves of sugarcane (Saccharumsp., variety Co 1007) are cultivatedin vitroon a synthetic medium. The first anatomical modifications appear in nonlignified, subepidermal fibers, on the external side of the leaves, by the 3rd day. Cellular growth precedes proliferation. Thereafter the process of dedifferentiation affects all of the tissues of the leaves. Proliferations of epidermal tissue, subepidermal fibers, bundle sheath cells, and vascular tissue give rise, respectively, to the epidermal tissue, peripheral cambial zone, parenchyma, and vascular tissues of the calluses. Thus, organogenic specificity of each foliar tissue is preserved.
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