The Reverse Transcriptase Codon 69 Insertion Is Observed in Nucleoside Reverse Transcriptase Inhibitor-Experienced HIV-1-Infected Individuals, Including Those Without Prior or Concurrent Zidovudine Therapy

摘要

Objectivescolon;This study was undertaken to examine 6-bp insertions following codon 69 in the reverse transcriptase (RT) coding region of human immunodeficiency virus type 1 (HIV-1) mutations in terms of incidence, presence of additional RT mutations, phenotypic drug resistance, HIV-1 RNA levels, and antiretroviral treatment history.Study Designsol;Methodscolon;A retrospective study of 121 nucleoside reverse transcriptase inhibitor (NRTI)-experienced subjects infected with HIV-1 was performed. Methods included quantitation of HIV-1 RNA levels, genotypic analyses of the RT and protease coding regions, and determination of phenotypic drug resistance.Resultscolon;A 6-bp insertion following RT codon 69 was observed in viral isolates from 4 subjects. Two subjects had a history of zidovudine (ZDV)-based therapy, and two subjects had a history of stavudine (D4T)-based therapy without prior exposure to ZDV. The T69S mutation and the 6-bp insertion following RT codon 69 were the only RT mutations observed in the 2 subjects with a history of D4T-based therapy.Conclusionscolon;Six-basepair insertions occurred in virus from 4 of 121 (3percnt;) NRTI-experienced subjects, including those without prior ZDV treatment, and was observed in the absence of the T215Y mutation. There was no apparent correlation between insertion incidence and HIV-1 viremia.Journal of Human Virology 1999;2:290-295 copy; Lippincott Williams amp; Wilkins, Inc.