In vitro studies of floral shoot apices ofPharbitis nil

摘要

Shoot apices ofPharbitis nilwere excised and cultured in vitro at different intervals after the intact plants were photoperiodically induced. The induced apices excised immediately after and up to 24ensp;h after an inductive dark period, grew vegetatively for at least 4 weeks in culture. The apices excised and cultured from intact plants at 36 and 48ensp;h after induction initiated a full complement of floral-organ primordia when observed after 4 weeks of growth.