Protein analysis and electron microscopic observation of the freeze-fractured plane of the plasma membrane were performed with an acriflavine-sensitive mutant carrying mutationacrA(at min 10) and with the wild type (acrA+) strain ofEscherichia coliK-12. TheacrAmutant membrane was deficient (or much lower) in one protein when analyzed by the polyacrylamide gel electrophoresis technique.
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