Subzelluläre Verteilung von Enzymen des Purinabbaues in pflanzlichen Speicherorganen

摘要

The distribution of urate oxidase (E.C. 1.7.3.3), allantoinase (E.C. 3.5.2.5), and allantoicase (E.C. 3.5.3.4) was investigated in subcellular fractions from potato tubers and from cotyledons of germinating seeds of pumpkins and peas. Urate oxidase and allantoinase were detected in all three plants, allantoicase only in potatoes. The isolation and characterization of glyoxysomes from pumpkin cotyledons and of peroxisomes from pea cotyledons is described. In all three tissues a considerable part of the urate oxidase activity was present in the peroxisomes, which contained also a significant part of the allantoinase activity of pea and pumpkin seeds. The rest of the enzyme activities was detected in the 10 000gsupernatant of the differential centrifugation.