Isometric virus-like particles encapsidate the double-stranded RNA found inPuccinia striiformis,Puccinia recondita, andPuccinia sorghi

摘要

Isolates ofPuccinia striiformis(wheat stripe rust),Puccinia recondita(wheat leaf rust), andPuccinia sorghi(maize rust) contain complexes of double-stranded RNA. In each species, there are at least 12 double-stranded RNAs ranging in size from 150 to 5000 base pairs, and the number, size, and quantitative relationships between the double-stranded RNAs are distinctive for a given species. Most, if not all, of the double-stranded RNAs in each species can be extracted from isometric virus-like particles of 40ensp;nm in diameter purified by sucrose and (or) Cs2SO4gradients. The data indicate that in these three species ofPucciniathe double-stranded RNAs are encapsidated in virus-like particles.