Changes in the Pattern of Proteins Synthesized in the Shoot Apical Meristem ofSinapis albaduring Floral Transition

摘要

BecauseSinapis albacan be induced to flower by a single long day (LD), we have sought for changes in gene expression in the shoot meristem during the floral transition induced by a 22 h LD. To analyse the pattern of proteins synthesized in the meristem, we have used in vivo labelling of proteins, an improved extraction procedure specifically designed for green plant tissues, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and a strict gel comparison procedure giving a confidence level of 3.Following the LD, complex qualitative and quantitative changes affected about 8of the proteins. Twelve different patterns of changes were observed. Some quantitative changes and one qualitative change were already found 16 h after the divergence of light regimes, other changes appeared only later. Except for one protein, the qualitative changes appeared only after the production of flower primordia.