Effect of variety and plant growth regulators on callus proliferation and regeneration response of three tomato cultivars (Lycopersicon esculentum)

摘要

The experiment was conducted to optimize a reproducible protocol for callus induction and regeneration of three tomato cultivars and also to select the cultivar which better perform under In vitro conditions for further experimentation. For callus induction hypocotyls and leaf discs were used as explant source. Explants of the tomato seedlings were cultured on MS medium supplemented with different concentrations and combinations of different plant growth regulators (PGRS) for callus proliferation. Callus induction values were significantly influenced by the variety and explant source. In all three tested cultivars maximum Callus induction frequency was observed on CIM6 (MS + 0.5 mg/l IAA + 2mg/l 2ip). There was also a positive correlation between the treatment and explant source. Here at T6 (2ip 2mg/l, IAA0.5 mg/l) hypocotyls gave its maximum value of (81.8), followed by theT4 (IAA 1 mg/1 + kinetin 0.5 mg/l) for hypocotyls (79.48). To observe the regeneration capacity of three tomato cultivars, 10 Regeneration Media (RM) combinations were tested. The regeneration capacity is significantly influenced by cultivar and explant type. The best regeneration media was found to be RM3 (MS + 1.5 mg/l 2ip and 0.5 mg/l IAA) with (72.5-79.22) regeneration. Among varieties Riogarande showed maximum regeneration capacity (79.22).