Role of interferon‐gamma in the modulation of the IgE response by 2,4‐dinitrophenyl‐Bordetella pertussisvaccine in the mouse

摘要

AbstractImmunization of mice with 2,4‐dinitrophenyl‐Bordetella pertussis(DNP‐BP) failed to induce anti‐DNP IgE responses. Administration of DNP‐BP induced, however, the formation of anti‐DNP IgE B memory cells, as demonstrated by adoptive transfer. Furthermore, mice pretreated with DNP‐BP and primed with 2 μg DNP‐ovalbumin (OA) in alum 2 weeks later produced high day‐7 anti‐DNP IgE levels. These subsided to near undetectable levels by day 12–14. The transient expression of serum IgE levels was accompanied by normal levels of anti‐DNP IgG. The anti‐OA response induced as a result of priming with DNP‐OA in alum was not affected by pretreatment with DNP‐BP. IgG subclass analysis revealed that mice pretreated with DNP‐BP had elevated levels of IgG2aand reduced levels of IgG1as compared to control (TNP‐keyhole limpet hemocyanin‐pretreated) mice. Treatment of mice with an anti‐interferon‐γ monoclonal antibody, shortly after immunization with DNP‐BP, not only reduced anti‐DNP IgG2alevels, but prevented the sharp anti‐DNP IgE decline that occurred after priming with DNP‐OA in alum. These results suggest that DNP‐BP‐induced interferon‐γ production modulates I