The effects of heterologous antiserum Ig on the in vitro phagocytosis of SRBC by guinea pig peritoneal macrophages were studied. In vitro treatment of cultured peritoneal macrophages with ALS, ATS and AMS under tissue culture conditions inhibited their capacity to phagocytize opsonized SRBC; however, where treatment was carried out at room temperature this effect was not observed. Treatment of macrophage monolayers with heterologous antiserum Ig uniformly resulted in pronounced pseudopodia formation. Treatment of monolayers with AMS Ig at 37deg;C led to striking alterations of cell membranes and aggregation of cells. These observations suggest a form of in vitro macrophage activation.
展开▼
