Isoelectric Focusing and Two-Dimensional Gel Electrophoresis in Plasma and Cerebrospinal Fluid from Patients with Dementia

摘要

IgG profiles of cerebrospinal fluid (CSF) and plasma from patients with Alzheimer’s disease/senile dementia of Alzheimer type, multi-infarct dementia and aged nondemented individuals were analyzed using isoelectric focusing (IEF) followed by immunofixation and high resolution two-dimensional polyacrylamide gel electrophoresis (PAGE). The IEF profiles of plasma and CSF in patients with dementia were diffuse and lacked oligoclonal bands and were identical to those of nondemented individuals. The investigation of plasma and CSF by two-dimensional PAGE did not reveal any specific protein pattern in the demented individuals compared to the control