Studies on Low Crossabilities Encountered with theHordeum bulbosumMethod for Haploid Production of Barley,Hordeum vulgareL.

摘要

AbstractCrosses were made between four spring barley (Hordeum vulgareL.) cultivars and five F1, hybrids with one genotype ofHordeum bulbosumL. in two locations to investigate further previous low crossabilities which had been found in the barley cultivar ‘Apex’ withH. bulbosum.Data at all the main steps of theH. bulbosumtechnique were recorded andstatistically analyzed.Significant differences between barley genotypes were demonstrated for all characters. It was confirmed that ‘Apex’ has poor crossability withH. bulbosum.Out of the three F1hybrids having ‘Apex’ as one parent, two exhibited low crossability similarly to ‘Apex’ but one showed significantly higher seed setting than ‘Apex’.The effect of the location was only significant on seed setting, while genotype X location interactions were significant on seed setting, seed quality and rate of haploid plants in relation 10 florets pollinated. Another problem which has influenced the success rate of theH. bulbosummethod was found in the cultivar ‘Havilla’. Although seed setting and seed quality were high for this cultivar, embryo differentiation was low. However, this latter problem was found to influence less the overall success rate than poor crossability.Mahalanobis's distances were calculated and the dendrite of the shortest distances between barle