Indigenous production of synthetic seeds in Daucus carota

摘要

Embryogenic callus Culture of carrot (Daucus carota L.) was induced from the hypocotyl explants in MS medium supplemented with Casein hydrolysate, 2,4-D and BAP for somatic ernbryogenesis In vitro cell suspension Cultures for high multiplication rate. Embryogenic callus was transferred to liquid basal Murashige and Skoog medium The mature somatic embryos were encapsulated in a Sodium alginate mixture to produce synthetic seeds. Sodium alginate 2 and Calcium chloride 75mM was best for encapsulation. Germination frequency of encapsulated somatic embryos was evaluated upto 10 weeks successfully.