Influence of methyl isocyanate on the growth, ATP levels, and nitrogen fixation ofAzotobacter chroococcum

摘要

Growth, ATP levels, and N2-fixing activity ofAzotobacter chroococcumculture exposed to 500 μg ml-1of methyl isocyanate were investigated. In methyl isocyanate-exposed cells, the lag phase was extended up to 16 h but the control cells started to multiply immediately. ATP levels and N2-fixing activity as determined by acetylene reduction assay were markedly reduced in the treated cells