Production of embryogenic callus from nonembryonic explants of Norway SprucePicea abies(L.) Karst

摘要

Callus has been induced on needle and bud explants from 2, 6 and 26 year oldPicea abies(L.) Karst, trees. Seventy seven percent of the needle explants from the youngest material was productive compared to 0.2 for the old tree. New embryogenic sections appeared in the non-embryogenic callus derived from 2, 6 and 26 year old trees after prolonged culture and was promoted by the use of an established embryogenic callus line in a nurse culture system.