OPTIMUM OPERATING CONDITIONS OF BIOREACTOR SYSTEM FOR TRYPTIC HYDROLYSIS OF CA-CASEINATE

摘要

With an aim of improving the hydrolysis of Ca-caseinate in a practice, the immobilization method of trypsin tailored to the tryptic hydrolysis to obtain the caseinophosphopeptide was investigated, Covalent binding method with chitosan as a carrier was found to be superior to the physical adsorption method in terms of enzyme loading, relative activity of enzyme remained and reusability of enzyme, A packed-bed reactor, 0,01m in inside diameter, filled with immobilized trypsin, 0,08m in height, was devised to investigate the optimum operational conditions for hydrolysis of Ca-caseinate by employing the Response Surface Method, The optimum operational conditions searched were X-1 (recycle ratio) = 0.24, X-2 (substrate concentration) 2.28 (w/v), X-3 (pH) = 8.28 and X-4 (temperature) 37.7 degrees C. Under these conditions, the degree of hydrolysis (D.H,) was calculated to be 9.66 which is close to the theoretical value, 10.6. Experimentation referring to these conditions resulted in the 9.3 of D.H. References: 16