The effects of intragastric or intraduodenal instillation of 12 (v/v) ethanol upon exocrine pancreatic secretion were assessed in 9 mongrel dogs provided with pancreatic and gastric fistulae (Thomas cannula) and submitted to a continuous intravenous injection of GIH secretin (1.0 U/kg/h). The experiments were carried out on the same group of dogs either in the conscious state or under very light fluothane anesthesia. Intragastric instillation of ethanol (1.0 g/kg) in anesthetized dogs produced: (1) a significant increase in the flow rate and bicarbonate output equivalent to one quarter of the effect obtained by adding 1.0 U/kg/h of GIH secretin to the hormonal injection; (2) a significant increase in the protein concentration and output equivalent to one third of the effect obtained by adding 1.0 CHR unit of CCK to the secretin injection. Intraduodenal ethanol (0.7 g/kg) only changed protein concentration significantly in anesthetized dogs but did not induce any significant modification in conscious animals. Intraduodenal instillation of HCl (2.5 mmol) at the end of the ethanol infusion revealed that the intestinal mucosa was still able to release secretin and CCK.
展开▼
