WhenPhanerochaete chrysosporiumdegrades 14C lignin-labelled aspen wood in static liquid culture, it releases almost as much14C in water-soluble form as in14CO2. Water-soluble ultraviolet-absorbing materials also accumulate in cultures that are degrading lignin. The water-soluble radioactive materials appear to be intermediates in lignin degradation, because they can be further metabolized to CO2and because the soluble materials labelled during 14C lignin degradation are distinct from the materials labelled during 14C glucose metabolism. These lignin degradation products can be fractionated by gel chromatography and are mostly acidic.
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