High performance size‐exclusion chromatography of anionic polymers in aqueous solutions

摘要

AbstractThe applicability of high performance size‐exclusion chromatography was tested for anionic polymers with a new type of column. It was found that a solvent salt concentration of 0.1Mand an elevated temperature (60°C) prevented adsorption of the polystyrene sulfonate standards on the column packing. The calibration curve and the effect of the concentration on the retention volume remained, however, colum‐dependent. We concluded also that the use of the column was restricted to the linear range of the calibration curve and to concentrations below 1 mg mL−1for the highest molecular weight standards. The influence of the flow rate (below 1 mL min−1) on the retention volume was negligible. The main cause of errors when the universal calibration technique is used originated in the experimental determination of the intrinsic viscosities of the standards. The combination of the errors on the viscosity and on the experimentally determined retention volume easily introduced an error of 15 on the determined molecular weight of the sulfated polysaccharidek‐carrageenan. The use of the universal calibration method for an exact molecular weight determination of anionic natural polymers is therefore still qu