Effect of guinea‐pig purified immunoglobulin G1 on the responsiveness of tracheal, aortic, vas deferens and ileum smooth muscles

摘要

SummaryBackgroundSmooth muscles hyperresponsiveness is a common feature in anaphylaxis and allergic diseases.ObjectiveThe aim of the present work was to investigate the effectof in vitropassive sensitization with highly purified immunoglobulin GI (IgGl) on the responsiveness of tracheal, aortic, vas deferens and ileum smooth muscles.MethodFirstly, IgGl, obtained from actively sensitized BFA guinea‐pigs, was purified by Protein A‐Sepharose column and characterized by enzyme‐linked immunosorbent assay (ELISA) and immunoelectrophoresis analysis. Concentration‐response curves to spasniogens (acelylcholine for trachea and vas deferens, noradrenaline for aorta and histamine for ileum) were established before and afterin vitropassive sensitizalion with IgGl.ResultsContractile responses and maximal contractions were significantly enhanced after passive sensitization for all the organs. Maximal contractions were significantly increased in the trachea (+46.7), aorta (+51), vas deferens (+114.2) and ileum (+ 117.2). At the end of the experiments, the application of the sensitizing antigen induced a significant Schultz‐Dale reaction of the smooth muscles.ConclusionThe present results show that thein vitroapplication of purified IgGl can produce non‐specific smooth muscle hyperreactivity and hypersensitivity. So, IgGl can be considered as the main factor involved in the genesis of sensitization‐induced hyperresponsiveness, and probably play a great role in hyperreactivity observed during allergic diseases an