A previous paper in this series has reported on the effect of bisectingAquilegiafloral apices of different developmental stages by maintaining a continuous photographic record of living buds (Jensen 1971). The current paper is a preliminary light and electron microscope study of the cytological changes which occur following bisection of very young buds (sepal to petal stages). Bisected buds show a progressive increase in size of grains which are positive to periodic acid ndash; Schiff stain up to 2 days after incision, followed by a progressive decrease in size up to 7 days after incision. These grains are probably starch as they were absent in sections treated with alpha;-amylase and malt diastase. It is suggested that after bisection the buds are in a resting state for 1 to 2 days during which time sucrose is absorbed from the medium and stored as starch. As regeneration of a new apex proceeds, starch is digested and used by the cells of the growing bud.
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