The in vitro simulation of IAA-induced modification ofAvenacell wall polysaccharides by an exo-glucanase

摘要

Treatment ofAvenacoloeptile segments with auxin promotes a decrease in the noncellulosic glucose component of the cell walls. The decrease in glucan may be simulated in these cell walls in vitro by an exo-glucanase which produces glucose directly as the sole reaction product. These results also reveal that glucans ofAvenacell wall polysaccharides are readily susceptible to exo-enzyme attack. Nojirimycin, a potent inhibitor ofβ-glucosidases and exo-glucanases, inhibits the enzymatic release of glucose fromAvenacell walls in vitro. Since nojirimycin inhibits IAA-induced growth ofAvenacoleoptiles, the evidence presented supports the hypothesis that IAA-induced growth may be mediated by an exo-glucanase