Synthesis and characterization of water-soluble two-photon excited blue fluorescent chromophores for bioimaging

摘要

We report here the synthesis and characterization of a new type of non-ionic blue fluorescent water-soluble chromophores specifically designed for two-photon absorption microscopy.The water solubility is induced by introduction of short oligo(ethylene glycol) monomethyl ether moieties.This work has led to low molecular weight dyes with efficient two-photon absorption cross sections and high fluorescence quantum yield in organic solvents as well as in aqueous solutions.