In vitro exposure of spleen cells to fetal calf serum prior to extensive washing and injection into syngeneic recipients was found to markedly increase in vitro responses to both trinitrophenyl-Ficoll and sheep erythrocytes, increase in vitro spontaneous (background) direct plaque-forming cell responses, and cause a tenfold increase in spontaneous IgM production in vitro. This study demonstrates T cell mediated polyclonal B cell activation which was manifested both in vitro and in vivo.
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