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>Mechanism of Photoregulated Carotenogenesis inRhodotorula minutaIII. Effect of Certain Nucleic Acid and Protein Inhibitors on Photoinduction of Carotenoid Biosynthesis
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Mechanism of Photoregulated Carotenogenesis inRhodotorula minutaIII. Effect of Certain Nucleic Acid and Protein Inhibitors on Photoinduction of Carotenoid Biosynthesis
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机译:Mechanism of Photoregulated Carotenogenesis inRhodotorula minutaIII. Effect of Certain Nucleic Acid and Protein Inhibitors on Photoinduction of Carotenoid Biosynthesis
Inhibitors of protein and nucleic acid synthesis such as puromycin, cycloheximide, 5-fluorodeoxyuridine and 5-fluorouracil have been used to study the dark reactions involved in photoregulation of carotenogenesis inRhodotorula minuta. The results indicated that as already reported in other organisms, carotenogenic enzymes are synthesized first and, in turn, synthesize carotenoids in the dark. Synthesis of the carotenogenic enzymes was absolutely dependent on oxygen and came to an end within 6 hr at 26°C under aerobic conditions. Photoregulation of this synthesis may occur at the translational level. 5-Fluorodeoxyuridine and 5-fluorouracil acted as chemical inducers of carotenogenesis inRh. minutagrown in the dark. However, the site of the action of these chemicals was assumed to be different from that of light, because the chemical and light effects on the induction of carotenogenesis were additive
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