Patients A and M developed allergic symptoms when working in a greenhouse with paprika plants and freesia plants, respectively.The possible involvement of an IgE-mediated mechanism was investigated with the skin prick test, radioallergosorbent test (RAST) and the histamine release test (HRT). Paprika flower, leaf and stem extract released 58, 47 and 43 of the total amount of histamine from washed leukocytes of patient A. In serum A IgE antibodies against paprika leaves and flowers could be demonstrated by RAST (11 binding of 125I-anti-IgE added). Freesia flower and stem extract released 46 and 43 histamine, respectively, from washed leukocytes of patient M. In the RAST, specific IgE antibodies against freesia flowers and stems were found in serum M (37 binding of 125I-anti-IgE added).
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