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首页> 外文期刊>Journal of optical technology >Using digital off-axis holograms to investigate changes of state of living neuronal cultures
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Using digital off-axis holograms to investigate changes of state of living neuronal cultures

机译:使用数字离轴全息图研究活神经元培养物状态的变化

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摘要

Apparatus has been developed and assembled to obtain holograms of a living cell culture by methods of digital and analog holography. The setup demonstrates the advantages of both methods and provides convenient adjustment and monitoring of its parameters for recording a series of digital holograms. Software processing of the results makes it possible to obtain information concerning the total optical thickness of the test samples and to visualize and measure changes in living neuronal cultures. Variations of the structure and manifestations of vital activity of a primary culture of brain cells have been investigated and compared.
机译:已经开发和组装了通过数字和模拟全息方法获得活细胞培养物的全息图的装置。该设置展示了这两种方法的优点,并提供了方便的调整和监控其参数,以记录一系列数字全息图。通过软件处理结果,可以获取有关测试样品总光学厚度的信息,并可视化和测量活神经元培养物的变化。已经研究和比较了脑细胞原代培养物的结构和生命活动表现的变化。

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