A Comparison of theBritish Pharmacopoeia(1973) andUnited States Pharmacopeia(1975) Methods for Detecting Pseudomonads

摘要

TheBritish Pharmacopoeia(Anon. 1973) test for contamination by pseudomonads and theUnited States Pharmacopeia(Anon. 1975) test for the detection ofPseudomonas aeruginosahave been evaluated using type cultures representative of a wide spectrum ofPseudomonasspp. Media containing cetrimide are recommended by both Pharmacopoeias for the detection of pseudomonads. Of the 28 strains tested only five were detected by theBPmethod and 11 by theUSPmethod. However, only one strain ofP. aeruginosafailed to be detected using theUSPmethod. A correlation between m.i.c. values for cetrimide and detection of the organisms was noted. TheBPmethod failed to detect organisms with m.i.c. values of ± 0.03 whilst theUSPmethod did not detect strains with m.i.c. values ± 0.015