Organ culture with black cottonwood: morphogenetic response of female catkin primordia

摘要

Female catkin primordia of black cottonwood (Populus trichocarpaT. amp; G. ex Hook.) were cultured for 70 days on a modified Murashige and Skoog's (1962) medium in vitro. Explants 2ndash;3ensp;mm long, and with bud scales removed, gave the best results, many of them developing floral structures characteristic of the female sex. There was a general tendency to callus formation with increasing age of the culture, occasionally followed by a reversal to vegetative growth. Catkin primordia raised on Wolter's medium without auxin or kinetin, but with 6-benzylaminopurine, and at 250ensp;ft-c for a 16-h photoperiod, proliferated axillary shootsin locoof pistils.