An amylose-grafted chitosan has been synthesized by a chemoenzymatic method according to the following two reactions. First, maltoheptaose is introduced to chitosan by a reductive amination using sodium cyanotrihydroborate in a mixed solvent of 1.0 mol center dot L-1 aqueous acetic acid and methanol at room temperature to produce a maltoheptaose-grafted chitosan that has a well-defined molecular structure. A phos phorylas e-catalyzed enzymatic polymerization of alpha-D-glucose 1-phosphate is then performed from the maltoheptaose-grafted chitosan to obtain the amylosegrafted chitosan. This material does not dissolve in any solvent, e.g., aqueous acetic acid and dimethyl sufoxide, which are good solvents for chitosan and amylose, respectively.
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