Transgenic plants of rutabaga (Brassica napobrassica) tolerant to pest insects

摘要

Cotyledons cut from axenic seedlings were immersed inAgrobacterium tumefacienssuspension which was treated with acetosyringone and nopaline at low pH overnight. The infected cotyledon explants were cultured on MSB medium (MS salts + B5Vitamins) containing 6-BA 3mg/1 for 2–3 days, and transferred onto selective medium (MSB with kanamycin 50–100 mg/l). Kanamycin-resistant shoots were selected. More than 60 regenerated plants were obtained. About 60 of the plants showed high NPT II activity. Southern blot hybridization showed that some of the plants gave a positive signal with the insecticidal crystal protein gene (cry IAgene) probe, and exhibited tolerant to insects such asPieris rapae(cabbage caterpillar) in leaf feeding experiments. Kanamycin-resistance and insect-resistance were maintained in the prog