Reversed Phase Ion-Pair Chromatographic Separation of Acetyl Choline and Choline

摘要

A reversed phase High-Performance Liquid Chromatographic procedure for the separation of choline and acetyl choline is described. The method employs a mobile phase containing aromatic sulphonates as counter ions for ion-pair chromatography and for U.V. Visualisation of choline and acetyl choline at 254 nm. The method enables the separation of the two compounds in the 1-10 ug concentration range in six minutes. The separations are better achieved on a PRP-I resin column.