Med15B Regulates Acid Stress Response and Tolerance in Candida glabrata by Altering Membrane Lipid Composition

摘要

Candida glabrata is a promising producer of organic acids. To elucidate the physiological function of the Mediator tail subunit Med15B in the response to low-pH stress, we constructed a deletion strain, C. glabrata med15B Delta, and an overexpression strain, C. glabrata HTU Delta/CgMED15B. Deletion of MED15B caused biomass production, glucose consumption rate, and cell viability to decrease by 28.3, 31.7, and 26.5, respectively, compared with those of the parent (HTU Delta) strain at pH 2.0. Expression of lipid metabolism-related genes was significantly downregulated in the med15B Delta strain, whereas key genes of ergosterol biosynthesis showed abnormal upregulation. This caused the proportion of C-18:1 fatty acids, the ratio of unsaturated to saturated fatty acids (UFA/SFA), and the total phospholipid content to decrease by 11.6, 27.4, and 37.6, respectively. Cells failed to synthesize fecosterol and ergosterol, leading to the accumulation and a 60.3-fold increase in the concentration of zymosterol. Additionally, cells showed reductions of 69.2, 11.6, and 21.8 in membrane integrity, fluidity, and H+-ATPase activity, respectively. In contrast, overexpression of Med15B increased the C18: 1 levels, total phospholipids, ergosterol content, and UFA/ SFA by 18.6, 143.5, 94.5, and 18.7, respectively. Membrane integrity, fluidity, and H+-ATPase activity also increased by 30.2, 6.9, and 51.8, respectively. Furthermore, in the absence of pH buffering, dry weight of cells and pyruvate concentrations were 29.3 and 61.2 higher, respectively, than those of the parent strain. These results indicated that in C. glabrata, Med15B regulates tolerance toward low pH via transcriptional regulation of acid stress response genes and alteration in lipid composition.