An indirect immunoferritin-labeling technique is used to localize cell surface immunoglobulin (Ig) allotypic determinants on rabbit lymph node, bone marrow and thymus lymphocytes. 47 of the lymph node cells, 62 of bone marrow small lymphocytes and less than 1 of thymic lymphocytes, are positive for surface Ig. Two Ig-positive lymph node lymphocyte populations which differ in fine structure are identified but both cell types express similar amounts of surface Ig (7,000–28,000 Ig molecules per cell). Bone marrow small lymphocytes make up only 9 of the total cell population and express less surface Ig (ca. 3,000–12,000 molecules Ig per cell) than most Ig-bearing lymph node lymphocytes. Two types of morphologically distinct thymus lymphocytes are described and both are essentially negative for surface Ig using this labeling techni
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