Determination of 4‐methylpyrazole in plasma using solid phase extraction and HPLC

摘要

AbstractA rapid method for the determination of 4‐methylpyrazole in plasma is described. Internal standard (3‐methylpyrazole) is added to the plasma which is subsequently applied to a BondElut SCX column. After washing the column the 3‐ and 4‐methylpyrazoles are eluted with a phosphate buffer and analyzed by isocratic reversed‐phase high performance liquid chromatography with UV detection. The concentration of 4‐methylpyrazole is calculated from the 4‐methylpyrazole/3‐methylpyrazole peak high ratio. The method is linear between 2.5 and 100 μmol/L with a within‐day precision (CV) of 2.2 (n= 10) and a day‐to‐day precision of 2.8 (n= 30). The sensitivity is sufficient for analysis of plasma levels in th