Abstract.This study reports the adaptation of an electronic particle‐counting method for the enumeration of T cell and B cell rosettes of human lymphocytes. This method and the conventional hemocytometer method were compared in parallel on seventeen T cell and seven B cell rosette enumerations in a blind study design. All data were analyzed for statistical significance; correlation coefficients were calculated. There was significant agreement between both methods for Tcells at p<0.001 (mean particle counter 80.6 ±11.3, mean hemocytometer 76.7 ± 8.2) and for the B cells at p<0.002 (mean particle counter 22.5±9.7, mean hemocytometer 23.4±5.12). These results demonstrate that the electronic particle counter is useful as a rapid, convenient, semi‐automated method for the enumeration of T cell and B cell r
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