Tobacco mosaic virus specific immunoglobulins from horse serum. I. Physical and chemical characterization

摘要

AbstractIgG and IgG(T) immunoglobulin fractions have been prepared from an anti‐serum of a horse which had been immunized with tobacco mosaic virus. The virus specific antibodies were isolated by precipitation with the virus followed by dissociation of the precipitates. The molecular weights and the carbohydrate contents of the immunoglobulins and of their constituent polypeptide chains were determined. Neutralization tests with TMV and IgG showed that the neutralizing capacity resides primarily in the H‐chains. The peptide chains of IgG(T) exhibited a greatly reduced neutralizing effect as compared to the IgG(T) antib