Recombination has been detected for the first time between chromosome 6 loci controlling kappa chain expression in normal mouse serum immunoglobulin and theLyt-3locus. The recombination event occurred at the 26th or 27th backcross generation during the derivation of theLyt-2a,Lyt-3a-congenic line B6.PL(85NS). The line is now homozygous for theLyt-2a,Lyt-3aallele(s) at N30F13 and homozygous animals express theIgk-Ef1ballele derived from C57BL/6. The frequency of recombination has been estimated to be 0.30 based on the present results and previous studies in which no recombination was detected. The results rule out the hypothesis that theLyt-3locus itself controls the light chain phenotype observed in normal serum immunoglobulin.
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