Identification ofPseudomonasspp. Isolated from Milk Produced in South Eastern Queensland

摘要

Summary.Bacteria were isolated from raw and pasteurized milks produced throughout S. E. Queensland. Milk samples were plated initially on penicillin agar or on milk agar and incubated at 30° and 7°, respectively. On the basis of primary characterization, 167 of the 330 isolates obtained were identified asPseudomonasspp. The pseudomonads were further characterized in accordance with the taxonomic studies of the genus by Stanier, PalleroniDoudoroff (1966). Species designations were ascribed to thePseudomonasisolates on the basis of distinctive species characteristics in conjunction with similarity coefficients between each isolate and the ideal species phenotype, as follows:Ps. fluorescens(121),Ps. aeruginosa(16),Ps. putida(12),Ps. maltophilia(9),Ps. pseudoalcaligenes(5),Ps. cepacia(3) andPs. alcaligenes(1). A dendrogram obtained by cluster analysis of thePseudomonasisolates is include