Changes in Endogenous Amino Acid Compositions during Somatic Embryogenesis inDaucus carotaL.

摘要

Changes in amino acid compositions of carrot cells cultured in three different media were examined in relation to somatic embryogenesis. The total amount of amino acids in the 80ethanol-insoluble fractions increased rapidly during cell proliferation and globular embryo formation. The individual proportions of the amino acids in these fractions remained fairly constant irrespective of the development of the somatic embryos. The total amount of amino acids in the 80ethanol-soluble fractions varied depending on the medium used.Continuously appliedα-alanine did not accumulated in the cells, which suggests that it was transformed to other amino acids during culture. A large amount ofγaminobutyric acid was detected throughout the culture period. Glutamic acid and glutamine accumulated in the carrot cells during embryo formation and maturation. Changes in the compositions and amounts of the endogenous amino acids present during embryo formation are discusse