Energy requirements for protein translocation across theEscherichia coliinner membrane

摘要

SummaryBoth ATP and an electrochemical potential play roles in translocating proteins across the Inner membrane ofEscherichia coli.Recent discoveries have dissected the overall transmembrane movement into separate subreactions with different energy requirements, identified a translocation ATPase, and reconstituted both energy‐requiring steps of the reaction from purified components. A more refined understanding of the energetics of this fundamental process is beginning to provide answers about the basic issues of how proteins move across the hydrophobic membrane barrie