Determination of the Mono and Diethanolamides of Palmitic Acid and of Soybean Oil Fatty Acids by High Performance Liquid Chromatography

摘要

HPLC separations and quantitative analyses are described for palmitic and soya monoethanolamides and diethanolamides synthesized from free fatty acids, methyl esters and triglycerides. Both reverse phase and adsorption techniques were employed, using a differential refractometer for detection. Starting materials and crude reaction products are analyzed without treatment or preliminary separations. The methods described are simple and rapid and can be used to monitor the course of condensation reactions as well as final products compositions. In some instances separation of homologous fatty acid alkanolamides was achieved, enabling comparison of the yields obtained from individual fatty acid precursors.