Investigations were made to determine the nature of a reducing substance which was formed on illuminating a reaction system consisting of chloroplast and cytoplasmic fraction of spinach leaves. From the results of spectrophotometric examinations it was concluded that the photoproduct in question, or at least its major portion, was ascorbic acid. The precursor of ascorbic acid, which was found to be heat unstable, was contained in the cytoplasmic fraction.
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