Numerical analysis of electrophoretic protein patterns ofMorganella morganiistrains from faeces, wound, urine and other clinical sources

摘要

Sixty‐five strains ofMorganella morganii(mainly of human origin) were characterized by one‐dimensional SDS‐PAGE of cellular proteins. The strains came from various countries; 13 were from stools (including one from a toucan), 13 from wounds, 11 from urine, five from blood (including one from a snake), five from the respiratory tract (four sputum, one lung), 12 from miscellaneous sources and six from unknown sources. The protein patterns, which contained 45 to 50 discrete bands, were highly reproducible. The patterns of 67M. morganiicultures plus those of the type strains of sevenProteusandProvidenciaspecies were used as the basis for two numerical analyses. In the first, which included all the protein bands, theM. morganiistrains formed 21 clusters at the 91 S level. In the second analysis, in which the principal protein bands (in the 31.6—43.2 kDa range) were excluded, the 67M. morganiicultures formed a single cluster at the 80 S level distinct from the sevenProteusandProvidenciareference strains. We conclude that high resolution PAGE combined with computerized analysis of protein patterns provides the basis for typing clinical strains ofM. morganii. Reference strains of each of the 21 PAGE types identified are available from NCTC for inclusion in future