Immunoregulation of lysozyme‐specific suppression. II. Hen egg‐white lysozyme‐specific monoclonal suppressor T cell factor suppresses the afferent phase of delayed‐type hypersensitivity and induces second‐order suppressor T cells

摘要

AbstractCulture supernatant from a monoclonal T cell lymphoma line (LH8‐105) obtained by radiation leukemia virus‐induced transformation of hen egg‐white lysozyme (HEL)‐specific suppressor T lymphocytes is able, when injected into mice, to specifically suppress the delayed‐type hypersensitivity (DTH) reaction induced by HEL. The suppressor T cell factor (TsF) exhibits fine antigenic specificity since it suppresses the DTH response induced by HEL without affecting the DTH response induced by ring‐necked pheasant egg‐white lysozyme (REL), a lysozyme closely related to HEL. Conversely, LH8‐105 TsF is able to suppress the DTH response induced by human lysozyme, distantly related to HEL but sharing a common epitope critical for induction of suppressive activity. The fine antigenic specificity of LH8‐105 TsF for a restricted epitope on the HEL molecule is confirmed by binding to HEL but not to REL immunosorbents. This TsF also bears I‐J determinants, as demonstrated by binding to monoclonal anti‐I‐J immunosorbents, and it suppresses the afferent but not the efferent phase of the DTH response to HEL. The afferent suppression is controlled by genes apparently mapping in the I‐J subregion of the H‐2 complex since I‐J‐incompatible mice are not suppressed by LH8‐105 TsF injection. This inducer‐type TsF induces second‐order effector suppressor T cells only in HEL‐primed mice indicating the primary role of antigen, in association with H‐2 (I‐J) products, in the a