Effects of Carbon Dioxide, Air Flow Rate, and Inoculation Density on the Batch Growth ofCatharanthus roseusCell Suspensions in Stirred Fermentors

摘要

AbstractA high reproducibility of batch growth ofCatharanthus roseuscould only be achieved by using identical inoculum material. It was found that the initial growth rate of the cells increased with increasing inoculation density, whereas the maximum growth rate was not affected. Withdrawal of the conditioned medium from the inoculum resulted in a strong decline in the initial growth rate, indicating that conditioning factors affected the initial growth rate. Neither increasing the air flow rate from 0.5 to 1.5 volume of air per volume of broth‐min−1(vvm) nor CO2enrichment of the aeration gas had any effect on the growth of the ce