Modulation of Lymphocyte and Monocyte Responses in vitro by 9-Deoxy-Δsup9/sup-prostaglandin Dsub2/suband 9-Deoxy-Δsup9/sup-Δsup12/sup-prostaglandin Dsub2/sub

摘要

The effects of 9-deoxy-Δ9-prostaglandin D2 (PGJ2) and 9-deoxy-Δ9-Δ12-prostaglandin D2 (Δ12PGJ2), which are metabolites of PGD2, on lymphocyte and monocyte reactions were studied in vitro. Expression of various phenotypic markers of lymphocyte subsets, as detected by monoclonal antibodies, was not affected by overnight incubation in 3 × 10–5M PGJ2. Phytohemagglutinin stimulation and natural killer activity of lymphocytes was reduced by PGJ2 and Δ12 PGJ2. Monocyte reactivity to 12-o -tetradecanoylphorbol-13-acetate, as assessed by chemiluminescence, was stimulated by preincubation of the cells for 1 h in 3 × 10––5 M PGJ2 or Δ12 PGJ2. Such an augmentation was not exerted by PG